A versatile flow-based assay for immunocyte-mediated cytotoxicity
نویسندگان
چکیده
منابع مشابه
Rapid flow cytometry-based assay for the evaluation of γδ T cell-mediated cytotoxicity
The effector function of natural killer, lymphokine--activated killer cells and T lymphocytes is commonly evaluated by radioactive chromium‑release cytotoxicity assays. In addition to this indirect method, fluorescence assays have been described for the assessment of in vitro cell‑mediated cytotoxicity. In the present study, target cells were stained with 5‑(and‑6)‑carboxyfluorescein diacetate ...
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Assessment of natural killer cells (NK-cell) cytotoxicity is used not only in research settings but is also important in diagnosis of various diseases. NK-cell cytotoxicity assays are based on measurement of target cells killed by cytotoxic cells analyzed either by chromium (51Cr) release assay or flow cytometry. Both these methods use peripheral blood mononuclear cells (PBMC) or pure NK-cell p...
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ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 2019
ISSN: 0022-1759
DOI: 10.1016/j.jim.2019.112668